Human FAM186B ELISA Kit (HUFI04338)

FAM186B (Family with Sequence Similarity 186 Member B), also referenced by its chromosomal alias C12orf26, encodes a human protein of largely unknown function that has surfaced as a candidate biomarker in transcriptomic and proteomic surveys of tumor and immune cell populations. This Assay Genie ELISA provides researchers with a quantitative tool to measure endogenous FAM186B protein levels in multiple types of human biospecimens including serum samples and plasma specimens, supporting studies in oncology biomarker discovery, immune signaling pathway characterization, and serum proteome mapping projects where orthogonal antibody-based validation of mass spectrometry findings is required. The kit ships with a pre-coated microplate, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, and substrate solution sufficient for 96 individual determinations in one assay run. Each plate is sealed in foil pouches with desiccant and should remain refrigerated at 4 degrees C until first use; reconstituted standard should be aliquoted to limit freeze-thaw cycles, and the working detection antibody solution is intended for single-use preparation on the day of the assay. Researchers running this kit commonly pair it with orthogonal validation methods such as western blot or qPCR to confirm tissue-specific expression patterns of FAM186B observed in publicly available databases like GTEx and the Human Protein Atlas.