Human FAM105A ELISA Kit (HUFI04358)

The Assay Genie immunoassay kit targets human FAM105A, the OTU family deubiquitinase now annotated as OTULIN that selectively hydrolyzes linear Met1-linked ubiquitin chains and modulates innate immune signaling through the NF-κB and Wnt pathways. Investigators studying OTULIN-related autoinflammatory disease, linear ubiquitin chain biology, and tumor necrosis factor receptor signaling use this kit to quantify FAM105A protein in cell culture supernatants and clarified lysates prepared from cultured human lines.

Each kit ships as a complete, ready-to-use package containing pre-coated microplate strips, calibrated reference standards, biotinylated detection reagent, streptavidin-HRP conjugate, wash concentrate, substrate solution, and stop solution. The assay follows a standard quantitative immunoassay workflow performed in vitro at the bench, with absorbance read on a standard plate reader. Reconstituted reagents and diluted samples are typically refrigerated for short-term use, and unopened components are stored refrigerated to preserve activity through the stated shelf life.

Intended for research use only, this kit is supplied in a microplate format that supports multiple replicates per plate, with sufficient reagents for the listed number of determinations per kit. For precise run setup and lot-specific performance characteristics, consult the package insert that ships with the kit from Assay Genie.