Human FAIM3 (Fas Apoptotic Inhibitory Molecule 3) ELISA Kit (HUES01591)

The TOSO/FcμR protein is the only known high-affinity Fc receptor specific for IgM, distinguishing this assay from generic apoptosis or B-cell marker panels. FAIM3 encodes a transmembrane glycoprotein of approximately 60 kDa on lymphocytes, while an alternatively spliced soluble isoform of roughly 40 kDa circulates in serum and is markedly elevated in chronic lymphocytic leukemia patients. The Assay Genie kit follows a sandwich double-antibody protocol with a biotinylated detection antibody and streptavidin-HRP/TMB readout measured at 450 nm, delivering a typical dynamic range of 0.156–10 ng/mL with sensitivity near 0.06 ng/mL. Reported precision falls within intra-assay CV below 10% and inter-assay CV below 12%, and no significant cross-reactivity with homologous analogues has been documented. Researchers tracking immune regulation can pair the quantitative ELISA with flow cytometry, immunohistochemistry, or western blot to localize FAIM3 expression on CD19+ B cells, CD4+ and CD8+ T cells, and CD56+ NK populations, and to study SYK-mediated BCR signaling crosstalk relevant to autoimmune disease and transplant tolerance. Unopened kits are shipped refrigerated; once opened, lyophilized standards, biotinylated antibody, streptavidin-HRP, and TMB substrate should remain refrigerated at 2–8°C and used within the kit's shelf life, while wash buffer and stop solution tolerate room temperature (around 20–25 °C) storage.