Human FABP2 PharmaGenie ELISA Kit (SBRS0547)

An absorbance-based immunoassay from Assay Genie enables quantitative measurement of human intestinal Fatty Acid Binding Protein 2 (FABP2 / I-FABP), a small cytosolic ~15 kDa fatty-acid carrier released by enterocytes when mucosal integrity is compromised. The 96-assay format is frequently used in research on mesenteric ischemia, necrotizing enterocolitis, inflammatory bowel disease, celiac disease, and gut-barrier dysfunction models.

Assay performance is calibrated to detect soluble I-FABP in plasma, serum, and cell culture supernatant samples, supporting both translational biomarker work and in vitro epithelial cell experiments where fatty-acid transport or barrier-stress responses are studied. Reagents are supplied in individual vials, with the protocol calling for refrigerated storage between uses and reconstituted components handled to limit freeze-thaw cycles.

Researchers commonly pair the kit with intestinal organoid cultures, Caco-2 enterocyte models, and patient-cohort plasma panels tracking I-FABP kinetics after suspected mucosal injury. Output is read on a standard microplate reader, and the workflow is documented for research use only, not for diagnostic procedures. Setup is straightforward for laboratories already running ELISA workflows, with the plate layout, standard curve, and sample dilution scheme detailed in the product insert.