Human F7/Factor VII ELISA Kit

A colorimetric sandwich ELISA designed for quantitative detection of human Factor VII in research samples. Assay Genie supplies the kit with biotin-labeled detection antibody and HRP-streptavidin signal amplification, using TMB substrate read at 450 nm. The assay has a sensitivity of 18.75 pg/mL and a standard range of 31.25–2000 pg/mL, with reactivity confirmed against human samples including plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

The procedure uses primary incubation at 37 °C for 90 minutes, detection antibody binding for 60 minutes, and HRP-streptavidin binding for 30 minutes, followed by 10–20 minutes of TMB color development. Recovery in EDTA plasma (n=5) ranges from 85–100% with an average of 93%, and in heparin plasma (n=5) from 90–105% with an average of 96%. Factor VII (F7, Coagulation Factor VII, FVII, Proconvertin, SPCA; UniProt P08709) is a key component of the blood coagulation cascade, and the kit is positioned for research into hemostasis, thrombosis, and related cardiovascular conditions. Reagent storage is at 2–8 °C for 12 months.