Human Extracellular Signal Regulated Kinase 2 (ERK2) ELISA Kit

The Assay Genie ERK2 quantitative immunoassay provides researchers with a reliable tool for measuring Extracellular Signal-Regulated Kinase 2 (also called MAPK1) in experimental samples. ERK2 sits at the heart of the RAS-RAF-MEK-ERK signal transduction cascade, transmitting mitogenic cues from the cell surface to nuclear transcription factors that govern proliferation, differentiation, and survival programs.

This 96-assay configuration supports studies in oncology, neurodegeneration, and metabolic disease, where dysregulated MAPK signaling is a frequent focus. Researchers commonly apply the kit to investigations of tumor cell lines, neuronal cultures, and tissue homogenates in vitro, including western blot validation workflows and pathway-level pharmacology screens. Each kit is supplied as a complete set of reagents and standards prepared for research use only, with vials of capture antibody, detection antibody, reference standard, and substrate working solution packaged in the box.

Important handling notes: equilibrate reagents to room temperature before use, protect chromogen from light, and avoid repeated freeze-thaw cycles of prepared working solutions to preserve activity. The accompanying protocol recommends small sample aliquots of around 50 µL per determination so that residual material can be archived for follow-up assays.