Human Excitatory amino acid transporter 1 (SLC1A3) ELISA Kit

For neuroscience and neuropharmacology laboratories investigating excitatory amino acid signaling, Assay Genie offers a quantitative ELISA kit targeting human SLC1A3 (EAAT1). The transporter is the principal astrocytal glutamate uptake mechanism in the cerebellum, hippocampus, and cerebral cortex, and altered EAAT1 expression has been documented in amyotrophic lateral sclerosis, glioma, and excitotoxic injury models.

The kit accommodates a 96-assay microplate format with colorimetric readout, providing a practical balance between sample throughput and reagent economy for cohort-level studies. Each box contains a pre-coated microwell strip plate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, stop reagent, and a protocol booklet with sample preparation guidance.

Working with cell lysates, tissue homogenates, and conditioned media from cultured astrocytes, researchers can monitor SLC1A3 regulation in response to pharmacological challenge, gene silencing, or inflammatory cytokine exposure. Reagents should be equilibrated to room temperature before use and stored according to the manufacturer's instructions to preserve activity across the kit's shelf life.

By combining a sensitive immunoassay workflow with standardized calibration, this Assay Genie product helps laboratories generate reproducible SLC1A3 quantification data across multiple experimental batches and operator runs.