Human EV71-IgG (Enterovirus 71-Immunoglobulin G) ELISA Kit (HUFI08020)

Developed by Assay Genie for serological surveillance of Enterovirus 71, this Indirect-Biotin ELISA quantifies human IgG antibodies specific to EV71 capsid antigens. The kit supports research into hand, foot, and mouth disease epidemiology, post-infection immunity assessment, and EV71 vaccine immunogenicity studies.

A biotinylated secondary antibody paired with streptavidin-HRP amplification enhances signal generation for low-titre specimens, while the included standard curve enables reproducible interpolation of antibody concentrations. The protocol uses a sample volume of 50 µL per well, accommodating paediatric and limited-volume specimens. Each microplate supplies 96 Assays with all buffers, standards, and detection reagents provided ready-to-use.

The assay shows species-specific reactivity to human IgG and demonstrates no significant cross-reactivity with antibodies against related enteroviruses such as Coxsackievirus A16, supporting confident interpretation of EV71-specific immune status. For research use only; not intended for clinical diagnostic procedures.