Human Eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3) ELISA Kit

A sandwich ELISA kit from Assay Genie for the quantitative detection of human Eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), also known by the synonyms PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK, PEK, and PERK. The assay is intended for research use only and is reactive with human samples.

Validated sample types include serum, EDTA plasma, heparin plasma, tissue homogenates, cell culture supernates, and other biological fluids. Reported performance characteristics are a detection range of 0.312–20 ng/mL, a sensitivity of 0.15 ng/mL, an intra-assay CV of 6.0%, an inter-assay CV of 11.7%, and specificity for natural and recombinant human EIF2AK3. The indicated research area is Neurosciences.

EIF2AK3 is described as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on Ser-52 during the unfolded protein response and in response to low amino acid availability. The protein localises to the endoplasmic reticulum as a single-pass type I membrane protein, and UniProt accession Q9NZJ5 is referenced.