Human Estrogen receptor (ESR1) ELISA Kit

The ESR1 protein, also known as estrogen receptor alpha, is a ligand-activated transcription factor that mediates the cellular response to 17β-estradiol in target tissues such as breast, bone, uterus, and cardiovascular tissue. It is therefore a central biomarker in breast cancer research, osteoporosis studies, and investigations of hormone-dependent disease. This Assay Genie kit provides a quantitative immunoassay for measuring ESR1 levels in human samples, supplied as a complete pack containing a pre-coated microplate, matched antibody reagents, calibrated standards, wash buffers, and chromogenic substrate. The workflow follows standard ELISA practice: pipette samples and standards onto the plate, incubate, wash, add the detection antibody and substrate, then quantify the colorimetric signal on a microplate reader. All kit components should be refrigerated upon arrival, and samples are best stored as frozen aliquots to minimize repeated freeze-thaw cycles; bring reagents to room temperature before use. Researchers routinely apply this assay to studies of receptor expression, signalling pathway analysis, and screening of compounds that modulate estrogenic activity. The kit is intended for research use only and is not approved for diagnostic procedures.