Human ESPL1 (Separin) ELISA KIT (HUFI05264)

This Assay Genie Separase ELISA kit targets the ESPL1 gene product, a cysteine endopeptidase that cleaves the cohesin Scc1/RAD21 subunit to release sister chromatids at the onset of anaphase. The immunoassay supports research on chromosome segregation fidelity, mitotic checkpoint regulation, and aneuploidy in dividing cells.

Investigators studying breast, ovarian, and hepatocellular carcinoma use Separase quantification to explore how overexpression contributes to genomic instability and tumor progression. The microplate-based format includes pre-coated wells, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, and a recombinant standard to build a calibration curve.