Human ESM1/Endocan ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit employs a double-antibody method with colorimetric detection to quantify human ESM1 (Endocan) in research samples. ESM1 is a protein implicated in inflammation, endothelial cell function, and angiogenesis; elevated levels have been associated with inflammatory diseases, cancer, and cardiovascular disorders, making it a valuable biomarker for studying disease progression and therapeutic interventions.

The assay delivers a sensitivity of 9.375 pg/mL and a standard range of 15.625–1000 pg/mL. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with kit components stored at 2–8°C for up to 12 months. Linearity in serum, EDTA plasma, and heparin plasma spans roughly 82–105% across 1:2 to 1:8 dilutions, while recovery averages fall between 96–97% for the same matrices.

The workflow proceeds through reagent equilibration, primary incubation at 37°C for 90 minutes, biotinylated detection antibody binding, HRP-streptavidin conjugation, TMB color development, and absorbance reading at 450 nm after stop solution addition. Provided reagents include a dismountable pre-coated microplate, lyophilized standard, biotin-labeled antibody concentrate, HRP-streptavidin conjugate, TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, wash buffer, stop solution, plate sealers, and a technical manual.