Human Erythrocyte membrane protein band 4.2 (EPB42) ELISA Kit

Designed by Assay Genie, this Sandwich ELISA Kit is intended for the quantitative measurement of human Erythrocyte membrane protein band 4.2 (EPB42), also known by the synonyms Erythrocyte protein 4.2 and E42P. The assay uses a colorimetric ELISA detection method with reactivity for human samples, and is suitable for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Performance characteristics quoted from the product specification include a detection range of 0.156-10 ng/mL, a sensitivity of 0.098 ng/mL, an Intra-CV of 4.6% and an Inter-CV of 6.5%. Specificity is reported for natural and recombinant human Erythrocyte membrane protein band 4.2. Recovery is stated as an average of 88% (range 82-94) for serum and 90% (range 84-96) for plasma.

EPB42 is described as probably playing an important role in the regulation of erythrocyte shape and mechanical properties. The protein is localised at the cell membrane (lipid-anchor, cytoplasmic side), cytoplasm, cytoskeleton and the cytoplasmic surface of erythrocyte membranes, and it interacts as an oligomer with the cytoplasmic domain of SLC4A1/band 3 anion transport protein. Defects in EPB42 are associated with spherocytosis type 5 (SPH5). The UniProt accession is P16452, the chromosomal location of the human ortholog is 15q15-q21, and the molecular weight is reported as 69,455 Da. The kit is for research use only.