Human ERK1/MAPK3 ELISA Kit

From Assay Genie, this Sandwich ELISA kit using a Double Antibody method is designed for the quantitative measurement of ERK1/MAPK3 levels in human samples. ERK1/MAPK3, also known as extracellular signal-regulated kinase 1/mitogen-activated protein kinase 3, is described as a key signaling protein involved in cell growth, proliferation, and differentiation, with dysregulation linked to diseases including cancer, inflammatory disorders, and neurodegenerative diseases. The kit is catalogued under the Cell Death research area.

The manufacturer states a sensitivity of 0.188 ng/mL and a detection range of 0.313–20 ng/mL. The kit is intended for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and should be stored at 2–8°C for 12 months. Reported linearity values (n=5) are: serum 89–103% at 1:2, 86–100% at 1:4, 89–99% at 1:8; EDTA plasma 86–103%, 94–99%, 87–99%; heparin plasma 94–104%, 87–98%, 90–98%. Recovery ranges and averages are: serum 86–105% (average 91%), EDTA plasma 86–103% (average 93%), and heparin plasma 89–104% (average 95%).

The assay procedure covers reagent and plate preparation, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding at 37°C for 60 minutes, HRP-Streptavidin binding at 37°C for 30 minutes, TMB substrate color development in the dark for 10–20 minutes, and a stop reaction with absorbance read at 450 nm. Kit components include an ELISA microplate, lyophilized standard, biotin-labeled antibody (100X), HRP-Streptavidin conjugate SABC (100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer (25X), plate sealer, and a technical manual.