Human Epithelial Stromal Interaction 1, Breast (EPSTI1) ELISA Kit

This quantitative immunoassay from Assay Genie measures EPSTI1 (Epithelial Stromal Interaction 1, Breast) protein levels in human-derived samples. EPSTI1 regulates epithelial-stromal cross-talk in mammary tissue and has been implicated in cell adhesion, immune signaling, and remodeling of the tumor microenvironment. Researchers studying breast cancer progression, stromal-epithelial communication, or mammary gland biology use this kit to quantify EPSTI1 expression in tissue homogenates and cell lysates.

Each kit supplies reagents sufficient for 96 assays in a microplate format with absorbance-based readout compatible with standard laboratory plate readers. The assay is validated for human reactivity and is intended for research use only. In the workflow, prepared standards and samples are added to pre-coated wells, followed by detection reagent and substrate; absorbance values are recorded and EPSTI1 concentrations are interpolated from a standard curve.

Sample preparation notes: cell lysates are prepared in RIPA buffer with added protease inhibitors, while tissue homogenates are produced by mechanical disruption in cold PBS. Store kit components refrigerated and protect from light; allow all reagents to equilibrate to room temperature before running the assay. For research use only — not for use in diagnostic procedures.