Human Epididymal Protein 4/HE4 ELISA Kit

A high-quality Sandwich ELISA from Assay Genie designed for the accurate and precise measurement of Human Epididymal Protein 4 (HE4) levels in human biological samples. The kit uses a colorimetric detection method and is reactive with human samples, supporting research on serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. HE4 is recognized as a key protein associated with ovarian cancer and has proven to be a valuable biomarker for the early detection and monitoring of the disease. Elevated HE4 levels have also been linked to other gynecological conditions, such as endometriosis and pelvic inflammatory disease.

The assay workflow uses a pre-coated plate: standards, samples and blanks are loaded and incubated at 37°C for 90 minutes to allow antigen binding, followed by a biotin-labeled detection antibody incubation for 60 minutes, HRP-Streptavidin (SABC) binding for 30 minutes, TMB substrate color development in the dark for 10-20 minutes, and absorbance measurement at 450 nm after stop solution addition.

With its dependable and consistent performance, the kit is intended as an essential tool for researchers and healthcare professionals seeking to advance understanding of ovarian cancer and related disorders.