Human Epidermal Growth Factor (EGF) ELISA Kit

This Assay Genie kit provides quantitative measurement of human Epidermal Growth Factor, a 6.2 kDa mitogenic polypeptide that activates EGFR signaling in epithelial and mesenchymal cells. Researchers commonly apply this kit to wound healing studies, mucosal repair investigations, oncology biomarker work, and tear fluid or saliva-based diagnostics research. The protocol pairs a biotinylated capture antibody with streptavidin-HRP detection on a pre-coated microplate, with absorbance read on a standard plate reader. Each well uses 100 µL of sample, and the kit includes recombinant human EGF standard calibrated against an internal reference. Reagents ship ready-to-use; store the sealed kit refrigerated, protect the streptavidin-HRP from light, and aliquot reconstituted standard to minimize freeze-thaw cycles. The pack contains one microplate, detection antibody, standards, and buffers sufficient for 96 assays. Research use only.