Human EPAC1 (Exchange protein directly activated by cAMP 1) ELISA Kit (HUFI07944)

Built around the cAMP-sensing EPAC pathway, this quantitative immunoassay from Assay Genie provides a research tool for measuring Exchange Protein Directly Activated by cAMP 1 in human-derived specimens. EPAC1, also known by the gene name RAPGEF3, operates as a Rap-family guanine nucleotide exchange factor and represents a distinct branch of cyclic AMP signaling that runs in parallel to protein kinase A. The kit supports investigations of vascular homeostasis, cardiac contractility, beta-adrenergic receptor signaling, insulin secretion, platelet aggregation, and other EPAC-linked cellular processes.

Supplied in a 96 Assays format with all reagents ready for immediate use, the kit is intended for research use only. Scientists studying G-protein coupled receptor cascades, cAMP compartmentation, or small GTPase regulation can apply it to profiling EPAC1 abundance in lysates and clarified homogenates prepared from human samples. The protocol terminates in an absorbance-based readout, enabling data capture on standard microplate readers equipped for ELISA-style quantification. Components should be kept protected from light and used within their shelf life to preserve capture antibody and reference standard performance.