Human Eotaxin PharmaGenie ELISA Kit (SBRS0532)

Built on the Assay Genie PharmaGenie platform, this kit supports reproducible quantification of CCL11 (Eotaxin), a small eosinophil-attracting chemokine implicated in asthma, allergic rhinitis, and eosinophilic inflammatory disorders. The workflow pairs pre-coated capture antibodies with biotinylated detection reagents and streptavidin-HRP for signal generation, read on a standard microplate reader. Reconstituted standards and lyophilised components are supplied in individual vials within the kit pack; unopened kits should be refrigerated, with working dilutions aliquoted and stored frozen to limit freeze-thaw cycles. The protocol accommodates cell culture supernatant, plasma, and serum sample inputs using 100 µL sample volumes per well, with intra-assay precision verified against recombinant human CCL11 calibrators (sensitivity below 5 pg/mL) and no significant cross-reactivity observed with murine CCL11, supporting biomarker profiling in allergy and immunology research.