Human Eosinophil Chemotactic Factor (ECF) ELISA Kit

The Assay Genie Human Eosinophil Chemotactic Factor (ECF) ELISA Kit quantitatively measures eotaxin (CCL11), an 8.5 kDa chemokine that drives eosinophil recruitment in allergic inflammation, asthma, and parasitic infection research. Reconstituted standards and detection antibody should be stored at -20°C, while unopened kit components remain stable at 2-8°C; avoid repeated freeze-thaw cycles by aliquoting the standard. The kit is supplied as a 96-well microplate pre-coated with human-specific capture antibody, paired with biotinylated detection reagent, streptavidin-HRP, TMB substrate, and calibrated standards. Researchers investigating airway inflammation, atopic dermatitis, helminth immunity, and eosinophilic esophagitis commonly use this assay with serum, plasma, cell culture supernatant, and tissue homogenate samples. Each kit supports research use only and includes a detailed protocol with intra-assay and inter-assay precision data for reliable quantification of CCL11 across biological matrices.