Human ENTPD8 ELISA Kit (HUFI04425)

From a structural standpoint, ENTPD8 belongs to the CD39-like ectonucleotidase family and is most abundantly expressed on the canalicular membrane of hepatocytes and along the apical brush border of intestinal epithelial cells, where it terminates extracellular purinergic signals by converting ATP and ADP to AMP. Researchers investigating cholestasis, bile acid transport, mucosal barrier integrity, and inflammatory bowel disease biology rely on ENTPD8 quantification to track tissue-level remodeling. The Assay Genie kit employs a biotinylated capture antibody paired with streptavidin-HRP signal generation, with liquid working reagents supplied in single-use vials to minimize cross-batch contamination. Reconstituted standards remain stable for roughly 7 days under refrigerated conditions, while the lyophilized recombinant reference tolerates extended storage at -20 °C. Typical run time is around 3.5 hours at room temperature, and the protocol accommodates blood-derived matrices with a recommended dilution step to neutralize matrix interference. Each pack contains one microplate and a sealed desiccant pouch housing the pre-coated strip wells. This product is intended for research use only and is not validated for diagnostic or therapeutic decision-making.