Human ENTPD4 ELISA Kit (HUFI04422)

Quantify human ectonucleoside triphosphate diphosphohydrolase 4 with this 96 Assays ELISA from Assay Genie, an in vitro research tool for studies of purinergic signaling, Golgi-resident nucleotide homeostasis, and intracellular phosphohydrolysis pathways. ENTPD4 belongs to the CD39/NTPDase family of ectonucleotidases that cleave triphosphate and diphosphate substrates, with altered expression reported across oncology, immune regulation, and glycosylation research. The kit ships with pre-coated microplate wells, calibration standards, detection antibodies, and substrate solutions sufficient for 96 Assays run side-by-side. Read on standard plate readers using colorimetric readout at the recommended wavelength, with standard curves generated from serial dilutions in supplied assay buffer. Storage guidance recommends refrigerating unopened components and aliquoting working reagents to limit freeze-thaw cycles. Sample handling covers clarified liquid biopsies and tissue-culture supernatants, with suggested dilution factors to keep unknowns within the linear working range. Lot-validated reproducibility, low inter-assay variability, and ready-to-run reagents make this ELISA a practical choice for labs profiling ENTPD4 transcript-protein concordance or screening modulators of nucleotide turnover in human disease models.