Human ENTPD2 ELISA Kit (HUFI04421)

Purinergic signaling research gains a quantitative tool with this Assay Genie ELISA kit for measuring human ectonucleoside triphosphate diphosphohydrolase 2 (ENTPD2), a cell-surface ectoenzyme also called CD39L1 that hydrolyzes extracellular ATP and ADP into AMP. ENTPD2 activity shapes extracellular nucleotide balance around endothelial cells, leukocyte populations, and tumor-stroma interfaces, making the protein a target of interest in vascular biology, inflammatory disease, and cancer microenvironment studies.

Configured as a 96 Assays kit, the assay provides enough reaction wells for complete standard curves, internal replicates, and matched disease-versus-control cohorts within a single run. Each kit ships with a pre-coated plate, calibrated recombinant standards, wash buffer, substrate solution, and stop reagent. The colorimetric readout is captured on a standard microplate reader, and the workflow is intended strictly for in vitro research use only, not for diagnostic procedures.

Typical laboratory applications include profiling ENTPD2 levels in patient liquid specimens, comparing expression between healthy and diseased cohorts, validating transcriptomic hits at the protein level, and screening candidate modulators of purinergic signaling pathways. Reagents remain stable under refrigerated storage through the kit's stated shelf life, and the protocol can be completed using standard immunobiology laboratory equipment.