Human eNOS/NOS3 PharmaGenie ELISA Kit (SBRS0529)

Endothelial nitric oxide synthase (eNOS, encoded by NOS3) is the vascular endothelial isoform of the nitric oxide synthase family that converts L-arginine to L-citrulline and nitric oxide in blood vessel linings. It governs vasodilation, angiogenic signaling, platelet aggregation, and vascular homeostasis, making it a central target in cardiovascular and metabolic disease research. Assay Genie's Human eNOS/NOS3 PharmaGenie ELISA Kit enables quantitative measurement of NOS3 protein in human research specimens across a 96-assay plate.

The kit supports quantification in serum samples, plasma specimens, and cell culture supernatant fractions, making it applicable to translational studies of endothelial dysfunction, atherosclerosis, hypertension, diabetes-related vascular complications, sepsis, and preeclampsia. Each kit supplies the pre-coated plate, detection reagents, calibrated protein standards, buffers, and substrate solution needed to complete one full plate of unknown samples interpolated against a standard curve.

For storage, unopened kits should be kept refrigerated, with components protected from light to preserve reagent activity. Researchers in vascular biology, pharmacology, and biomarker discovery rely on this plate-based protocol to monitor NOS3 expression changes in disease models and drug-response experiments, with the format supporting multiple replicates and dilution series for accurate quantification.