Human ENOPH1 ELISA Kit (HUFI04428)

Enolase-phosphatase 1 (ENOPH1), a bifunctional enzyme central to the methionine salvage pathway, catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate in eukaryotic cells. This enzyme underpins research into cellular sulfur metabolism, mitochondrial one-carbon cycling, and downstream metabolic regulation. Assay Genie offers a 96 Assays-format human ENOPH1 ELISA kit labeled for in vitro research use only, supporting quantitative measurement of native human ENOPH1 protein via a colorimetric readout compatible with standard microplate readers. Each kit includes a pre-coated microplate, assay diluent, detection antibody, wash buffer, substrate solution, and stop reagent in quantities sufficient for the stated number of determinations; reagents require refrigerated cold-chain handling until first use. Investigators studying metabolic enzyme regulation, methionine recycling in normal versus transformed tissue, or compound screening affecting ENOPH1 expression can apply the kit in a standard ELISA workflow with room-temperature incubation steps. The product ships as a complete packaging set with research use only labeling and is not cleared for diagnostic procedures; it supports academic, pharmaceutical, and biotechnology laboratory workflows that track native human ENOPH1 levels in cell and tissue lysates, conditioned media, and isolated blood fractions.