Human Enolase, Non Neuronal (NNE) ELISA Kit

The Assay Genie non-neuronal enolase (NNE) ELISA quantifies alpha-enolase (ENO1) in human specimens through a colorimetric microplate workflow intended for in vitro research use only.

Alpha-enolase is a ubiquitous glycolytic enzyme expressed across most tissues, distinct from neuron-specific enolase. Beyond its canonical metabolic role, ENO1 displays moonlighting functions on the cell surface, including plasminogen binding, which links it to fibrinolysis, immune modulation, and microbial pathogenesis. Researchers use this assay to investigate circulating alpha-enolase as a candidate biomarker in autoimmune disorders, certain malignancies, inflammatory conditions, and infectious disease studies where pathogen-derived enolase can elicit cross-reactive antibody or cytokine responses.

The kit supports 96 assays per microplate and supplies all reagents required to complete the colorimetric run. Investigators typically batch patient-derived samples and pair the resulting concentrations with clinical or experimental metadata to assess associations with disease activity, therapeutic response, or prognostic indicators across translational research projects.