Human ENO3 ELISA Kit (HUFI04426)

The Assay Genie Human ENO3 ELISA kit is a human-specific immunoassay enabling quantitative analysis of beta-enolase, a muscle-specific glycolytic enzyme classically studied as a biomarker for skeletal muscle injury, rhabdomyolysis, and inflammatory myopathy. The microplate configuration pairs a pre-coated capture antibody with an enzymatic readout readable on standard plate readers, and the human-specific design avoids cross-reactivity with rodent ENO3 paralogs, supporting clinical sample research and translational muscle studies.

Reconstituted standards should be aliquoted and kept frozen to minimize freeze-thaw loss of antibody binding activity, while unused strips remain refrigerated in their sealed foil pouch with desiccant. Each kit supplies components for a full microplate of independent determinations, supporting evaluation of muscle-derived biomarkers in serum and plasma specimens collected from research subjects. ENO3 is released from damaged myocytes into circulation following sarcolemmal disruption, making it a useful readout in neuromuscular disease research, exercise-physiology workflows, and cardiotoxicity screening. Research use only.