Human ENK (Enkephalin) ELISA Kit (HUFI04769)

Assay Genie's competitive ELISA kit enables quantitative measurement of endogenous human enkephalins, supporting neuroscience, analgesia, and neuropharmacology research workflows. Enkephalins are short endogenous opioid pentapeptides (Met-enkephalin and Leu-enkephalin) synthesized in the central nervous system and adrenal medulla that bind delta and mu opioid receptors to modulate nociception, stress responses, and reward pathways. Investigators studying pain transmission, opioid signaling, addiction biology, or neuroendocrine regulation rely on this in vitro immunoassay to evaluate enkephalin concentrations in experimental samples collected under approved study protocols. The competitive binding format allows sample peptide to compete with coated antigen for antibody binding, with bound antibody visualized through an enzymatic color development reaction quantified on a standard microplate reader. Reagents are supplied for 96 Assays in a break-apart well plate configuration, including pre-coated plate wells, peptide reference standards, detection conjugate, substrate solution, and assay buffer. For optimal peptide stability, reconstituted standards should be stored frozen and protected from repeated freeze-thaw cycles, while working dilutions remain stable refrigerated for short-term use. The kit is intended for research use only and is not validated for diagnostic or clinical applications.