Human Endoglin PharmaGenie ELISA Kit (SBRS0527)

Quantitative detection of soluble endoglin (CD105) in human research samples is supported by this Assay Genie microplate ELISA kit, which targets a 90 kDa homodimeric transmembrane glycoprotein functioning as an accessory receptor in the transforming growth factor-beta (TGF-β) signaling pathway. Endoglin is most strongly expressed on proliferating vascular endothelial cells, and its shed soluble form is widely investigated as a circulating biomarker of angiogenesis, tumor vascularization, endothelial dysfunction, and pregnancy-related vascular disorders such as preeclampsia.

The kit is supplied as a reagent set for 96 individual determinations in a strip plate format, with pre-coated wells, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, substrate solution, and assay buffers included. It accepts cell culture supernatant, human plasma, and human serum sample types, fitting both in vitro cell-based experiments and translational research workflows in oncology, cardiovascular biology, and reproductive medicine. The colorimetric end-point read-out is captured on a standard microplate reader, and the assay follows a quantitative two-site immunoassay design calibrated against a recombinant human endoglin standard curve for interpolation of unknowns.

Intended for research use only, the assay is suited to biomarker validation in pharmaceutical discovery, target engagement studies, characterization of endothelial cell models, and quantification of angiogenic factors in conditioned media or clinical research specimens. Each lot is QC-tested for intra- and inter-assay reproducibility, and the workflow fits standard laboratory schedules with minimal hands-on time.