Human Endo Beta-N-Acetylglucosaminidase (ENGASE) ELISA Kit

ENGASE (Endo-Beta-N-Acetylglucosaminidase) is a cytosolic glycoside hydrolase that cleaves the N,N'-diacetylchitobiose core of free N-glycans released from misfolded glycoproteins, playing a central role in the non-lysosomal degradation pathway studied in glycobiology and protein quality control research. The Assay Genie microplate format supports quantitative measurement of ENGASE protein levels in tissue homogenates and cell lysates, making this kit relevant for investigations into endoplasmic reticulum-associated degradation, N-glycan trimming, and cytosolic glycoprotein turnover. Researchers should aliquot reagents to limit freeze-thaw cycles and store the kit refrigerated at 4 °C, protected from light, to maintain stable performance across repeated runs. The pre-coated plate layout enables parallel processing of standards and unknowns, with each well accommodating a sample volume of 100 uL per the standard protocol. Intended for research use only, this ELISA supports comparative studies between human cell lines, primary tissue extracts, and conditioned media, allowing labs to profile ENGASE abundance in contexts ranging from neurodegeneration models to immune cell signaling experiments.