Human Emmprin/CD147 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit uses a double-antibody, colorimetric format for the quantitative measurement of human Emmprin/CD147, a cell-surface glycoprotein also known as Basigin (BSG) or Extracellular Matrix Metalloproteinase Inducer. The assay is intended for research use only and is designed for use with serum, plasma, cell culture supernatant, and cell or tissue lysate samples.

Performance characteristics include a detection range of 15.625–1000 pg/mL and a sensitivity of 9.375 pg/mL, with the capture antibody pre-coated onto the plate and a biotin-conjugated antibody used for detection. HRP-Streptavidin and TMB substrate drive a colorimetric readout measured at 450 nm, and the kit is shipped on ice packs with components stored at 2–8°C (with multi-month storage options for the standard and microplate at –20°C).

EMPRIN/CD147 is implicated in tumour invasion, metastasis, and inflammation, and the kit is suited to research into cancer, cardiovascular disease, and related conditions. Validated sample handling covers serum (allow blood to clot, centrifuge at 1000 × g for 20 minutes), EDTA or heparin plasma (centrifuge at 1000 × g for 15 minutes at 2–8°C), cell culture supernatant (centrifuge at 2500 rpm for 5 minutes), cell lysates prepared with PBS or mild RIPA, and tissue homogenates, with linearity and recovery data reported for serum, EDTA plasma, and heparin plasma matrices. The Uniprot reference for the target is P35613.