Human EMD (Emerin) ELISA KIT (HUFI08199)

Quantitative measurement of the nuclear envelope protein emerin is enabled by the Assay Genie human EMD ELISA kit, supporting translational studies of Emery-Dreifuss muscular dystrophy and broader laminopathy research. Emerin is a 254-amino-acid type II integral membrane protein anchored to the inner nuclear membrane, where it tethers chromatin to the nuclear periphery through interactions with lamin A/C and barrier-to-autointegration factor. Loss-of-function mutations in the EMD gene cause X-linked EDMD, a disorder characterized by early-onset joint contractures, progressive muscle weakness, and life-threatening cardiac conduction defects.

This research-use-only kit is supplied as a complete microplate package with all reagents needed to generate a standard curve and quantify emerin levels in human-derived specimens. The plate format accommodates batch processing of clinical and control cohorts, supporting studies that correlate circulating emerin concentrations with disease severity, genotype, or treatment response in muscular dystrophy patients. Beyond its primary indication, the kit can be applied to investigations of nuclear envelope remodeling during myogenic differentiation, cardiac tissue development, and cellular senescence.

Typical laboratory workflows involve running the ELISA plate to completion with paired standard dilutions and quality controls, then interpolating sample concentrations from the resulting absorbance profile. Reagents are intended for in vitro research applications and should be handled according to standard biosafety practices for human biological materials. Researchers are advised to consult the package insert for detailed protocol steps, reagent preparation, and recommended sample handling prior to first use.