Human EIF5B ELISA Kit (HUFI04441)

Human EIF5B (Eukaryotic Translation Initiation Factor 5B) is a GTPase that catalyzes joining of the 60S and 40S ribosomal subunits during translation initiation, and serves as the eukaryotic counterpart of prokaryotic IF2. The Assay Genie EIF5B ELISA Kit enables quantitative measurement of EIF5B protein levels with a standard curve range of 0.156–10 ng/mL, supporting investigations into translation control, ribosomal biogenesis, and cancer biology, where dysregulated expression of this initiation factor has been linked to tumor proliferation and poor prognosis across several malignancies. The kit includes a pre-coated 96-well microplate, biotinylated detection antibody, recombinant human EIF5B reference standards, streptavidin-HRP reagents, and assay buffer concentrate. Researchers commonly apply this kit to translational control studies, oncology biomarker discovery, and mechanistic investigations of eukaryotic initiation factor activity. Store refrigerated (2–8 °C) upon arrival, protect from light, and refer to the enclosed protocol for detailed assay procedure and recommended dilution guidelines.