Human EIF4G1 ELISA Kit (HUFI07952)

Quantify eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) in human research specimens using this colorimetric immunoassay from Assay Genie. EIF4G1 acts as a central scaffold within the eIF4F complex, bridging the cap-binding factor eIF4E and the DEAD-box helicase eIF4A during cap-dependent translation initiation. Proteolytic cleavage of EIF4G1 by viral 2A proteases during picornavirus infection dismantles host protein synthesis, making the protein a long-standing focus of host-pathogen research and a marker of translational dysregulation in cancer and stress response studies.

The kit is calibrated for use with serum or plasma specimens and supplies reagents for ninety-six individual determinations in a standard microplate layout, with a protocol readable on conventional absorbance plate readers. Researchers investigating mTOR-dependent translation control, IRES-driven translation under stress, ribosome recruitment, or viral host shutoff can use the assay to track full-length EIF4G1 and its cleavage fragments across experimental and clinical cohorts. Store kit components as indicated on each vial label; the kit is shipped under refrigerated conditions and is for research use only.