Human EIF3D ELISA Kit (HUFI04483)

The Human EIF3D ELISA Kit from Assay Genie enables quantitative measurement of eukaryotic translation initiation factor 3 subunit D (eIF3d) protein in research samples. EIF3D functions as a core subunit of the eIF3 multiprotein complex, which mediates cap-dependent translation initiation and ribosome recruitment to mRNA transcripts. This microplate-based immunoassay is relevant for studies on translational control, ribosome biogenesis, and the emerging role of eIF3D dysregulation in oncogenesis, including lung adenocarcinoma and hepatocellular carcinoma research models. Researchers add diluted specimen to pre-coated wells, incubate with a biotinylated detection antibody, and develop signal with streptavidin-HRP conjugate for absorbance readout. Standard laboratory precautions include refrigerating kit components upon arrival and avoiding repeated freeze-thaw cycles of reconstituted standards. Each kit provides sufficient reagents for the stated number of determinations and is intended for research use only, supporting applications such as biomarker discovery, translation factor profiling, and signaling pathway analysis in mammalian systems.