Human EIF3B ELISA Kit (HUFI04479)

EIF3B (Eukaryotic Translation Initiation Factor 3 Subunit B) serves as a scaffolding subunit of the eIF3 complex, the largest translation initiation factor, where it helps position the 40S ribosomal subunit on mRNA during cap-dependent protein synthesis. The Human EIF3B ELISA Kit from Assay Genie enables quantitative colorimetric measurement of EIF3B protein in human research specimens, with protocols accommodating serum-type samples or plasma-type preparations. Antibodies specific to EIF3B are immobilized on the plate; bound target is recognized by an enzyme-linked detection reagent that generates a chromogenic signal proportional to EIF3B abundance in each well of the microplate.

Each kit supplies reagents pre-configured for 96 assays, supporting medium-throughput investigation of translation control, ribosomal loading, and perturbations of cap-dependent protein synthesis. Useful research contexts include profiling EIF3B expression across tumor versus normal tissue panels, benchmarking changes following RNAi knockdown or pharmacological inhibition of the translation machinery, and surveying baseline levels in cultured cells or human biofluid specimens.

Store components refrigerated upon arrival; equilibrate the kit to room temperature before running the assay, and avoid repeated freeze-thaw cycles of the standard and sample buffers to preserve antibody activity across runs.