Human EIF2S3 ELISA Kit (HUFI04478)

This immunoassay from Assay Genie is built to quantify human EIF2S3, the gamma subunit of the heterotrimeric translation initiation factor eIF2 that delivers Met-tRNAiMet to the 40S ribosomal subunit during cap-dependent protein synthesis. EIF2S3 is encoded on the X chromosome (X-linked) and is essential for ternary complex formation; altered expression or mutation of the gene has been linked to the rare MEHMO syndrome (Mental retardation, Epileptic seizures, Hypogonadism and Genitalism, Microcephaly, and Obesity) as well as to integrated stress response signaling downstream of eIF2α phosphorylation.

Investigators working on translational control, neuron-specific translation defects, or stress granule biology can apply this 96 Assays ELISA to measure EIF2S3 levels in cell lysates and tissue homogenates prepared from cultured human cell lines. The plate is read by absorbance on a standard microplate reader, providing a quantitative comparison of EIF2S3 expression across experimental conditions, patient-derived samples, and knockdown or knockout controls.