Human EIF2AK3/PERK ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is configured as a Double Antibody immunoassay for the quantitative detection of human EIF2AK3/PERK protein. The assay is intended for human samples and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples [C0][S1]. Detection is colorimetric, with absorbance read at 450 nm after TMB substrate development [C0][S1]. EIF2AK3 (also known as eukaryotic translation initiation factor 2-alpha kinase 3, PERK, or pancreatic eIF2-alpha kinase; UniProt Q9NZJ5) is a key mediator of the unfolded protein response, and its dysregulation has been implicated in cancer, diabetes, and neurodegenerative disorders, supporting its use as a biomarker in metabolism research [S1].

Reported analytical performance includes a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL [S1]. Linearity (n = 5) across 1:2, 1:4, and 1:8 dilutions was 89–102% / 85–95% / 92–98% for serum, 93–105% / 86–97% / 91–100% for EDTA plasma, and 89–105% / 87–101% / 92–97% for heparin plasma [S1]. Spike-recovery (n = 5) averaged 97% for serum (range 87–94%), 90% for EDTA plasma (85–104%), and 94% for heparin plasma (88–98%) [S1]. The protocol comprises reagent equilibration, 37°C primary incubation for 90 minutes, biotinylated detection antibody for 60 minutes, HRP-Streptavidin for 30 minutes, TMB development for 10–20 minutes in the dark, and a stop reaction read immediately at 450 nm [S1]. Sample preparation guidance is provided for serum (clot, 1000 × g, 20 minutes), plasma (anticoagulant tube, 1000 × g, 15 minutes, 2–8°C), tissue homogenate, cell culture supernatant (2500 rpm, 5 minutes), and cell lysate [S1]. Unopened kits are stored at 2–8°C for 12 months [S1].