Human EIF1AX ELISA Kit (HUFI04482)

The Human EIF1AX ELISA Kit from Assay Genie enables quantitative measurement of eukaryotic translation initiation factor 1A, X-linked, a ~17 kDa ribosome-associated protein that plays a critical role in cap-dependent translation initiation and ribosome scanning. The capture antibody is specific for the human EIF1AX sequence, with no significant cross-reactivity with the paralog EIF1AY, supporting selective detection of the X-linked isoform. Quantitation is reported in ng/mL against a recombinant standard curve, and the assay is validated for use with standard human research sample matrices at a sample volume of typically 50-100 uL per well.

EIF1AX has emerged as a clinically informative biomarker in oncology research, particularly in uveal melanoma, thyroid carcinomas of the RAS-like molecular subtype, and subsets of ovarian and pituitary tumors where somatic hotspot mutations drive altered translational control. Researchers studying driver mutation co-occurrence with GNAQ, GNA11, or SF3B1 use the kit to evaluate protein-level changes that complement genomic profiling. The microplate-based workflow, ready-to-use reagents, and research-use-only designation make it a practical addition to translational and biomarker discovery laboratories investigating translation factor dysregulation in solid tumors.