Human EIF1 ELISA Kit (HUFI04487)

Quantitative detection of eukaryotic translation initiation factor 1 (EIF1) in research samples is supported by Assay Genie's Human EIF1 ELISA Kit. EIF1 is a conserved small protein that facilitates ribosomal subunit joining at mRNA start codons during protein synthesis, and altered expression has been associated with tumor biology and translation regulation studies.

Each kit contains a microplate pre-coated with capture antibody, biotinylated detection reagents, recombinant EIF1 standards for calibration curves, and assay buffers for the complete workflow. Researchers process cell lysates, tissue homogenates, and prepared biological specimens alongside reference standards. The kit supports in vitro research applications and is intended for research use only, not for diagnostic procedures.

Unopened kit components should be stored refrigerated until first use, with reconstituted standards aliquoted and frozen for repeated experiments. The protocol is read on a microplate reader, providing absorbance data for interpolation against the standard curve. Studies of oncogenic signaling, ribosome biogenesis, and X-linked gene expression use this kit to monitor EIF1 protein abundance in experimental models.