Human EEF2K (Eukaryotic elongation factor 2 kinase) ELISA KIT (HUFI08184)

The Assay Genie EEF2K ELISA Kit enables quantitative measurement of eukaryotic elongation factor 2 kinase levels in human serum and plasma samples. EEF2K (also termed Ca2+/calmodulin-dependent kinase III or CaMKIII) is a calcium/calmodulin-dependent kinase that phosphorylates eukaryotic elongation factor 2 on Thr56, reversibly inhibiting translational elongation during cellular stress, hypoxia, and nutrient deprivation.

Researchers use this human-specific capture antibody kit to investigate translational control in cancer biology, where EEF2K promotes tumor cell survival under metabolic stress, and to study neurodegenerative and metabolic signaling pathways linked to AMPK and mTOR. The microplate-based colorimetric format is intended for research use only and supports in vitro quantitative analysis across a full plate of individual determinations.

Store unopened kit components refrigerated and protected from light; equilibrate all reagents to room temperature before initiating the protocol. Reconstituted standards and detection antibody should be aliquoted and stored frozen to avoid repeated freeze-thaw cycles.