Human EED ELISA Kit (HUFI04512)

EED (Embryonic Ectoderm Development) is a core subunit of the Polycomb Repressive Complex 2 (PRC2), the methyltransferase responsible for depositing trimethyl marks on histone H3 at lysine 27. This quantitative immunoassay from Assay Genie enables researchers to measure EED protein levels in human specimens, supporting studies in developmental biology, epigenetic regulation, and oncology where dysregulated PRC2 activity has been linked to multiple cancer types.

The kit provides reagents for 96 determinations in a single use microplate format, including pre-coated wells, recombinant standards, a detection antibody, and substrate. Researchers apply this assay to compare EED expression across patient cohorts, tumor versus adjacent normal tissue, or treated versus untreated cell culture models. Results are read against a standard curve generated with the supplied calibrators.

Typical applications include basic research into polycomb-mediated gene silencing, biomarker discovery, and evaluation of small-molecule EED inhibitors under development as anticancer agents. Allow all components to reach room temperature before running the assay, and store the kit according to the instructions included with the shipment.