Human EDN/Eosinophil derived neurotoxin ELISA Kit

From Assay Genie, this sandwich ELISA kit uses a double-antibody format with colorimetric detection for the quantitative measurement of human Eosinophil Derived Neurotoxin (EDN). Reactivity is specific to human samples, and the kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reagents are stored at 2-8°C for 12 months.

Reported performance figures include a sensitivity of 0.375 ng/mL and a working assay range of 0.625-40 ng/mL. Linearity ranges across 1:2, 1:4, and 1:8 dilutions are 86-105% / 88-99% / 90-100% for serum, 85-99% / 87-97% / 85-94% for EDTA plasma, and 93-103% / 87-98% / 86-97% for heparin plasma (n=5 per matrix). Recovery ranges with averages are 93-105% (average 100%) for serum, 85-103% (average 94%) for EDTA plasma, and 85-103% (average 94%) for heparin plasma.

The procedure involves equilibrating reagents and TMB substrate to room temperature, incubating loaded wells at 37°C for 90 minutes, adding biotin-labelled detection antibody for 60 minutes at 37°C, adding HRP-Streptavidin (SABC) for 30 minutes at 37°C, developing with TMB substrate in the dark for 10-20 minutes, and reading absorbance at 450 nm immediately after stop solution. Sample handling includes clotting blood and centrifuging serum at 1000 × g for 20 minutes, or collecting plasma with anticoagulant and centrifuging at 1000 × g for 15 minutes at 2-8°C.