Human EDG7/LPAR3 ELISA Kit

Assay Genie offers a sandwich ELISA kit designed for the quantitative measurement of EDG7 (Lysophosphatidic acid receptor 3, also known as LPAR3) in human samples, using a double-antibody format. The assay is compatible with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Performance characteristics reported by the manufacturer include a sensitivity of 0.469 ng/mL and an assay range of 0.781-50 ng/mL. Linearity data (n = 5) are provided across 1:2, 1:4 and 1:8 dilutions for serum (88-103%, 87-102%, 85-98%), EDTA plasma (84-94%, 82-94%, 87-98%) and heparin plasma (81-96%, 82-100%, 80-90%). Recovery data (n = 5) show serum 87-102% (average 96%), EDTA plasma 89-102% (average 94%) and heparin plasma 85-104% (average 97%).

The procedure involves equilibrating reagents, loading standards and samples onto the pre-coated plate, incubating at 37°C for 90 minutes for antigen binding, adding a biotin-labelled detection antibody with a 37°C incubation for 60 minutes, adding HRP-Streptavidin for a 37°C incubation of 30 minutes, developing with TMB substrate in the dark for 10–20 minutes, stopping the reaction and reading absorbance at 450 nm. The kit is shipped on ice packs and components should be stored at 2-8°C (with some components suitable for -20°C storage), with a stated shelf life of 12 months. The kit includes a pre-coated microplate, lyophilised standard, biotin-labelled antibody (100X), HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample, antibody and SABC dilution buffers, stop solution, wash buffer (25X), Bradford reagent, plate sealer and a technical manual. A microplate reader with a 450 nm filter and a 37°C incubator are required but not supplied. The product is intended for research use only.