Human EDEM2 ELISA Kit (HUFI04522)

Endoplasmic reticulum-associated degradation (ERAD) is a critical protein quality control pathway that disposes of misfolded glycoproteins, and EDEM2 (ER Degradation Enhancing Alpha-Mannosidase Like Protein 2) plays a key recognition role in targeting these substrates for retrotranslocation and proteasomal destruction. This quantitative ELISA-format kit from Assay Genie enables researchers to measure human EDEM2 protein concentrations in research specimens, supporting investigations into ER stress, the unfolded protein response, and glycoprotein quality control mechanisms relevant to diabetes, neurodegeneration, and cancer biology.

The kit is supplied as a complete reagent set with a pre-coated microplate, detection antibodies, recombinant human EDEM2 standards, assay buffers, wash concentrate, and substrate, packaged for a full plate run. Each plate includes calibrators for generating a standard curve, and the procedure follows standard incubation and wash steps with absorbance readout on a compatible plate reader.

Investigators working on diabetes mellitus, neurodegenerative disease, viral infection, inflammatory signaling, and oncology can apply this kit to quantify EDEM2 levels across cell lysates, tissue homogenates, and other relevant biological matrices. It supports comparison of EDEM2 expression between healthy and diseased tissues, monitoring changes under pharmacological ER stress induction with compounds such as tunicamycin or thapsigargin, and characterization of cell lines or primary cells with altered ERAD capacity.

For optimal performance, store unopened kit components refrigerated at 4 degrees C, avoid repeated freeze-thaw cycles of diluted standards, aliquot reconstituted reagents when appropriate, and bring all components to room temperature before initiating the assay. Researchers should validate sample compatibility for their specific matrix, run appropriate positive and negative controls, and consult the product manual for species-specific cross-reactivity information before extending the assay to non-human samples.