Human EDA-A2 PharmaGenie ELISA Kit (SBRS0518)

Built for quantitative immunodetection of human ectodysplasin-A2 (EDA-A2), this colorimetric immunoassay kit from Assay Genie supports translational and basic research into the TNF-family ligand and its XEDAR receptor axis. Configured for 96 assays per plate run, it covers cell culture supernatant alongside plasma as well as serum specimens, and is calibrated against recombinant human EDA-A2 reference standards for accurate interpolation across the working range.

EDA-A2 is implicated in epithelial-mesenchymal signaling during embryonic ectodermal appendage formation, including hair follicle, tooth, and sweat gland morphogenesis. Altered expression has been studied in skin biology, tooth agenesis, X-linked hypohidrotic ectodermal dysplasia, and select tumor contexts. Researchers investigating keratinocyte behavior, ectodermal lineage specification, or inflammation-linked cytokine networks use this format to profile secreted EDA-A2 levels in conditioned media and clinical specimens.

The kit ships with a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, dilution buffer, wash concentrate, substrate, stop solution, and standards sufficient for the full plate. Reconstituted reagents should be stored refrigerated and protected from light. Aliquoted working stocks avoid repeated freeze-thaw cycles to preserve antibody activity. Refer to the kit insert for current storage temperature recommendations, working dilution guidance, and validated performance characteristics before initiating any run.