Human ECD ELISA Kit (HUFI04517)

The Human ECD ELISA Kit from Assay Genie targets ecdysoneless homolog, a conserved cell-cycle and RNA-splicing regulator also known as C20orf27 and SBP1, and uses a human-specific capture antibody to deliver quantitative readouts for translational research. Because altered ECD expression has been documented in breast, hepatocellular, and pancreatic tumor studies, the kit supports biomarker validation, cancer signaling investigations, and cell-proliferation assays where ELISA readouts are paired with western blot or PCR confirmation. Each kit supplies a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer, and serial-dilution standards sufficient to generate a full standard curve with sample replicates in the standard plate format. Working reagents are stored refrigerated until first use with unopened kits stable for several months; reconstituted standards should be aliquoted to limit freeze-thaw cycles and preserve assay reproducibility. Researchers typically load 100 uL of diluted sample and standards per well, run the incubation steps at room temperature, and read absorbance on a standard plate reader, following the included protocol for dilute-serum, EDTA-plasma, and heparin-plasma matrices.