Human EBLN1/Endogenous Bornavirus-like nucleoprotein 1 ELISA Kit

Assay Genie offers a sandwich ELISA, double-antibody immunoassay for the quantitative detection of human Endogenous Bornavirus-Like Nucleoprotein 1 (EBLN1). The kit targets the EBLN1 protein, also referred to as Endogenous Borna-Like N Element-1, and uses a colorimetric detection method.

The kit is reactive with human samples and accepts serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples. Its quoted sensitivity is 46.875 pg/mL with a working range of 78.125-5000 pg/mL. Linearity across dilutions has been demonstrated in serum (88-102% at 1:2; 85-104% at 1:4; 86-102% at 1:8), EDTA plasma (86-98% at 1:2; 85-101% at 1:4; 82-96% at 1:8) and heparin plasma (83-97% at 1:2; 85-100% at 1:4; 80-95% at 1:8). Spike-and-recovery averages are reported as 93% for serum, 94% for EDTA plasma and 92% for heparin plasma, with overall ranges of 85-105%, 85-105% and 85-100% respectively. The procedure comprises a 90-minute primary incubation at 37°C, a 60-minute incubation with biotin-labeled detection antibody, 30 minutes of HRP-Streptavidin binding, TMB substrate development in the dark for 10-20 minutes and absorbance readout at 450 nm. Components include a pre-coated microplate, lyophilized standard, biotinylated detection antibody concentrate, HRP-Streptavidin concentrate, TMB substrate, dilution buffers, wash buffer, stop solution and plate sealer. The kit should be stored at 2-8°C and is stable for 12 months under those conditions.