Human EBF3 ELISA Kit (HUFI04515)

The Assay Genie Human EBF3 ELISA Kit is intended for research-use-only quantification of Early B-cell Factor 3, a transcription factor in the Collier/Olf/EBF (COE) family with established roles in neuronal migration and B-cell lineage commitment. Mutations in the EBF3 gene are associated with Hypotonia, Ataxia, and Delayed Development Syndrome (HADDS), making this assay relevant for neuroscience and immunology laboratories investigating neurodevelopmental disease mechanisms and lymphocyte biology. Each kit provides reagents for 96 determinations using a sandwich immunoassay workflow with 100 uL sample volume per well and an absorbance-based readout compatible with standard microplate readers. Reconstituted standards and concentrated reagents should be stored frozen at -20 degrees C to preserve activity across multiple freeze-thaw cycles, while the antibody-coated plate and wash buffer remain refrigerated at 4 degrees C. The product supports biomarker validation, comparative expression studies in human serum and plasma, and translational research into transcription factor dysregulation in disease pathophysiology.