Human EBF1 ELISA Kit (HUFI04513)

This Assay Genie ELISA Kit enables quantitative measurement of Early B-cell Factor 1 (EBF1) in human specimens using a colorimetric method. EBF1 is a transcription factor central to B-cell lineage commitment, pre-B-cell differentiation, and neuronal development, and altered expression has been associated with B-cell acute lymphoblastic leukemia. Applications span lymphopoiesis, hematopoietic lineage specification, immune cell maturation, and transcription factor biology in cell culture and tissue-derived research samples. The 96 Assays configuration includes a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, recombinant protein standards, wash buffer, and substrate solution. Researchers generate a standard curve to interpolate unknown concentrations and process multiple samples in parallel for cohort or screening studies. Store unopened components refrigerated, protect conjugate and substrate from light, and equilibrate reagents to room temperature before starting the assay. This research use only kit supports investigation of EBF1 regulation across developmental, immunological, and disease contexts.